Animals
develop endogenous timing clocks called circadian rhythms (~24 hours) to adapt external environment. It
has been known that suprachiasmatic nucleus (SCN), located in hypothalamus in brain, is the central clock regulator. Circadian
rhythm is controlled by core clock genes – brain and muscle aryl hydrocarbon
receptor nuclear translocator (ARNT)-like protein 1 (Bmal1), circadian locomotor output cycles kaput (Clock), Period1/2 (Per1/2),
and Cryptochrome1/2 (Cry1/2),– among which Bmal1 and Clock act as mater regulators.
Although biological functions of circadian rhythm are well-studied in the SCN, the endogenous biological clock in peripheral tissues has not been fully elucidated. Several investigators have reported circadian rhythm in epithelial cell line2 and hair follicles3. Here, we report gene expression of the core clock genes rhythmically and dynamically changes in mouse skin through a day, indicating epithelial cells as well as dermal cells have their own rhythm. In addition, we show that transepidermal water loss (TEWL), a reflection of the skin barrier function, rhythmically changes throughout a day. Taken together, these results show that circadian rhythm in skin correlates with the physiological function of skin.
上記の結果を元に疾患モデルマウスを用いて同様の実験を行い、現在新たに得られた結果を加えたものを論文にすることを試みている。
Although biological functions of circadian rhythm are well-studied in the SCN, the endogenous biological clock in peripheral tissues has not been fully elucidated. Several investigators have reported circadian rhythm in epithelial cell line2 and hair follicles3. Here, we report gene expression of the core clock genes rhythmically and dynamically changes in mouse skin through a day, indicating epithelial cells as well as dermal cells have their own rhythm. In addition, we show that transepidermal water loss (TEWL), a reflection of the skin barrier function, rhythmically changes throughout a day. Taken together, these results show that circadian rhythm in skin correlates with the physiological function of skin.
上記の結果を元に疾患モデルマウスを用いて同様の実験を行い、現在新たに得られた結果を加えたものを論文にすることを試みている。
諸橋 啓太【第４学年】
